No Known Antidote: Quantification of a deadly toxin, tetrodotoxin, in red efts (Notopthalamus viridescens; Rafinesgue, 1820)
Mon, 04/29/2013 - 17:42
Abstract: Tetrodotoxin (TTX) in found in taxa worldwide including the eft life stage of the eastern newt (Notopthalamus virisdescens). Previous studies have shown that there is variability of TTX concentrations within efts and newts, and it is not known if the presence of TTX in these efts is common among populations. Our objective was to determine the distribution of TTX within the body N. viridescens efts. We hypothesized that the skin would have the highest concentrations of TTX as it is the area of the body that will likely come into contact with a predator. If certain regions of the skin come into contact with a predator more often than others, there may be significant differences in the dorsal, abdominal and pectoral regions of skin. Further, we hypothesized that the ova would have the second highest concentration of TTX as they are vital to reproduction yet vulnerable to predation. Through HPLC analysis we determined the concentrations of TTX within different tissues of N. viridescens efts including the: dorsal, pectoral, and abdominal skin; ovaries; testes; liver; pancreas; and alimentary canal. The concentrations of TTX between the different tissue types are not significantly different, nor were individuals significantly different in concentration of TTX from one another. Toxicity levels were high in comparison to other N. viridescens efts and more similar to the toxicity of highly toxic Taricha newts. The high toxicity of N. viridescens could be contributed to strong predation pressure by the slightly resistant garter snake (Thamnopsis sirtalis) and eastern hognose snake (Heterodon platirhinos).
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File Attachments: No Known Antidote Final Draft.docx
The Conservation and Management of Wolverine (Gulo gulo) Populations in Northern Idaho to Help Prevent Human Caused Extirpation from the Contiguous United States
Thu, 05/02/2013 - 11:21
Abstract: Wolverines (Gulo gulo) were once a thriving species in the North Western United States, but large scale trapping and poison programs in the early 1900s lead to the species near extinction. Since then, populations in the United States have been struggling to maintain a strong presence in Idaho. Its current listing as threatened on the Endangered Species Act prohibits hunting and trapping, but more management is needed to sustain populations. Human development and recreation activities have caused wolverines to disperse from its nature range. Using habitat preservation techniques on current and historical wolverine habitat, increase availability and connectivity will improve dispersal. Close relationship with state officials will provide protection regarding land use, recreation, hunting, trapping and harassment. Public education will teach residents ways they can help prevent wolverine populations from further decline. Extensive research and population monitoring are needed due to the currently declining populations and the low fecundity of the species.
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Major: Biology, Fisheries and Wildlife Science
File Attachments: Wolverine management in northern Idaho, U.S.
Monitoring the Zebra Mussel Invasion Front: Use of New Technology
Thu, 02/09/2012 - 15:39
Abstract: Zebra mussels are invasive mollusks that are affecting the well-being of the water bodies in the United States. This study uses environmental DNA (eDNA) is a sensitive early detection system that may be useful in monitoring their spread. The purpose of this study is to determine the effectiveness of eDNA technology in identifying infested water bodies, to determine if zebra mussel DNA is in the Adirondack water bodies not known to be infested, if the water chemistry of these water bodies is favorable for zebra mussel establishment, and if the eDNA technology is transferable to an institution like Paul Smith’s College. Eighteen lakes, all in New York State were sampled, fifteen of which are located in the Adirondack Park. DNA was extracted from water and plankton samples and species specific primers were used for PCR amplification to determine if zebra mussel DNA was present. Of seven samples taken from sites known to be infested, five of these tested positive for zebra mussel eDNA. Four lakes not known to be infested within the Park also tested positive for zebra mussel eDNA. Based on zebra mussel risk parameters (water chemistry) applied to 1,469 Adirondack water bodies, less than 3% are at risk of zebra mussel establishment. However it is possible that established populations could occur at microsites that may have locally high levels of calcium and higher pH.
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Major: Biology, Environmental Sciences, Environmental Studies, Fisheries and Wildlife Science, Forestry, Natural Resources Management and Policy
File Attachments: The Author has selected not to publish this complete work.