Tardigrade Abundance in Green Shield Lichens on Different Tree Species
Wed, 04/24/2013 - 18:43
Abstract: Many studies have been done on tardigrades, a microscopic, aquatic organism that feeds on plant cell fluid, bacteria, algae, protozoa, and other small invertebrates. Most of these studies have addressed their ability to survive extreme environments and not their preferred living habitats. Virtually no studies have been done investigating the ecology of tardigrades. This study focuses on which species of tardigrade live on a species of lichen (Common Greenshield Lichen, Flavoparmelia caperata) found on three species of trees; Red Maple (Acer rubrum), Black Cherry (Prunus serotina), and White Pine (Pinus strobus). Five trees of each species were chosen and five samples of the Common Greenshield Lichen were taken from all 25 trees during each of the fall and winter seasons. From every lichen sample processed, five slides were looked at, each containing two drops of the water that the lichen was suspended in for 2 hours. The samples were looked at underneath a compound microscope and a dichotomous key was used to identify tardigrades that were found. Due to the fact that liquid water is less available in winter, samples were taken during the fall and winter to look at the differences in species diversity and abundance. The greatest abundance of tardigrades was found on Red Maple, during both fall and winter. Black Cherry had the lowest abundance of tardigrades during both fall and winter. White Pine had an abundance less than that of Red Maple but greater than that of the Black Cherry. There were more tardigrades found on the lichen in fall than in winter. This implies that they find Red Maple a more suitable habitat than the Black Cherry and White Pine, may be related to acidity of the Black Cherry and White Pine being greater than that of Red Maple. Throughfall and stemflow on the trees may also contribute to habitat preference of the tardigrades.
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File Attachments: Capstone Report.docx
Monitoring the Zebra Mussel Invasion Front: Use of New Technology
Thu, 02/09/2012 - 15:39
Abstract: Zebra mussels are invasive mollusks that are affecting the well-being of the water bodies in the United States. This study uses environmental DNA (eDNA) is a sensitive early detection system that may be useful in monitoring their spread. The purpose of this study is to determine the effectiveness of eDNA technology in identifying infested water bodies, to determine if zebra mussel DNA is in the Adirondack water bodies not known to be infested, if the water chemistry of these water bodies is favorable for zebra mussel establishment, and if the eDNA technology is transferable to an institution like Paul Smith’s College. Eighteen lakes, all in New York State were sampled, fifteen of which are located in the Adirondack Park. DNA was extracted from water and plankton samples and species specific primers were used for PCR amplification to determine if zebra mussel DNA was present. Of seven samples taken from sites known to be infested, five of these tested positive for zebra mussel eDNA. Four lakes not known to be infested within the Park also tested positive for zebra mussel eDNA. Based on zebra mussel risk parameters (water chemistry) applied to 1,469 Adirondack water bodies, less than 3% are at risk of zebra mussel establishment. However it is possible that established populations could occur at microsites that may have locally high levels of calcium and higher pH.
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Major: Biology, Environmental Sciences, Environmental Studies, Fisheries and Wildlife Science, Forestry, Natural Resources Management and Policy
File Attachments: The Author has selected not to publish this complete work.